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<!--用于打印和文字放大--> <div class="content_tools_box"> <p class="content_tools"> <a href="javascript:CallPrint('print');">打印本文</a> <a href="javascript:doZoom(14)">小</a> <a href="javascript:doZoom(18)">中</a> <a href="javascript:doZoom(20)">大</a> </p> <div class="clearfix"></div> <script src="/template/D20170425/skin/js/c_tool.js" type="text/javascript" ></script> </div> <!--/用于打印和文字放大--> <!-- 正文 --> <div class="lead content" id="print"> <div style="white-space: normal; list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: 19.2px; vertical-align: baseline;">实验介绍</strong></span></div><p style="white-space: normal;"> <span style="font-family: 微软雅黑, "Microsoft YaHei";"> </span></p><p> 外泌体代谢组学研究是指通过对外泌体中代谢产物的测量和分析，来获得外泌体代谢状态的全面信息，从而探索外泌体在生物代谢中的作用和机制。外泌体代谢组学研究可以为研究人员提供关于外泌体代谢调节、疾病发生和发展、治疗策略等方面的信息。外泌体代谢组学技术服务项目通常包括外泌体的提取、代谢产物的分离和纯化、代谢产物的分析等多个环节。通过这些环节，研究人员可以获得外泌体代谢产物的全面信息，包括代谢产物的种类、含量、代谢途径等，进而对外泌体的代谢调节进行分析和研究。代谢组描述的是指生物体内源性代谢物质的整体，可以分为非靶向代谢组和靶向代谢组。</p><p><br/></p><p style="text-wrap: wrap;"><strong>1. 非靶代谢组</strong></p><p style="text-wrap: wrap;"> <span style="text-wrap: wrap;">外泌体非靶标代谢组学是一种基于orbitrap超高分辨质谱，针对外泌体这一样本类型能够实现代谢物准确定性的非靶向代谢组检测技术。一般是基于LC-MS技术对样本中的代谢物同时进行定性和定量分析，与本地自建库和商业标准品数据库同时进行一级、二级信息的匹配来进行物质鉴定。该方法适合检测大部分代谢物，技术成熟稳定，且适用于各种复杂生物样本，是研究代谢变化的主流技术。</span></p><p style="text-wrap: wrap; text-align: center;"><span style="text-wrap: wrap;"><img src="/upload/images/202304/16824064656255.jpg" title="16824064656255.jpg" alt="41.jpg"/></span></p><p style="text-wrap: wrap;"><strong>2. 靶向代谢组</strong></p><p style="text-wrap: wrap;"> 靶向代谢组学（Targeted metabolomics）是对指定列表的代谢产物的检测分析，特别针对一种或几种途径的代谢产物。分析的重点是采用大量天然和生物变异样本，验证预先确认的代谢物或已鉴定的潜在生物标记物。需要采用标准品进行准确的定性定量分析。高通量靶向代谢组可同时实现多种样本类型多种类物质检测，获得精确的定性定量结果，无需再次验证，特别适合发现代谢组学、临床转化和产业化研究。</p><p style="text-align: center;"><img src="/upload/images/202304/16824081065120.png" title="16824081065120.png" alt="42.png"/></p><p><span style="font-family: 微软雅黑;"><span style="font-size: 19.2px; background-color: rgb(255, 255, 255);"><strong><br/></strong></span></span></p><p><span style="font-family: 微软雅黑;"><span style="font-size: 19.2px; background-color: rgb(255, 255, 255);"><strong>检测项目</strong></span></span></p><p><br style="text-wrap: wrap;"/></p><table cellspacing="0" cellpadding="0" width="1105" align="center"><tbody><tr class="firstRow"><td width="103" style="border-top: 1px solid rgb(237, 125, 49); border-bottom: 1px solid rgb(237, 125, 49); border-left: 1px solid rgb(237, 125, 49); border-image: initial; border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务序号</span></strong></p></td><td width="255" style="border-top: 1px solid rgb(237, 125, 49); border-left: none; border-bottom: 1px solid rgb(237, 125, 49); border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务内容</span></strong></p></td><td width="558" style="border-top: 1px solid rgb(237, 125, 49); border-left: none; border-bottom: 1px solid rgb(237, 125, 49); border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务说明</span></strong></p></td><td width="125" style="border-top: 1px solid rgb(237, 125, 49); border-right: 1px solid rgb(237, 125, 49); border-bottom: 1px solid rgb(237, 125, 49); border-image: initial; border-left: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务周期</span></strong></p></td></tr><tr><td width="103" style="border-right: 1px solid rgb(244, 176, 131); border-bottom: 1px solid rgb(244, 176, 131); border-left: 1px solid rgb(244, 176, 131); border-image: initial; border-top: none; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">1</span></p></td><td width="255" style="border-top: none; border-left: none; border-bottom: 1px solid rgb(244, 176, 131); border-right: 1px solid rgb(244, 176, 131); background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">非靶代谢组</span></p></td><td width="558" style="border-top: none; border-left: none; border-bottom: 1px solid rgb(244, 176, 131); border-right: 1px solid rgb(244, 176, 131); background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">采用非靶代谢组方法进行检测并进行标准数据分析，搜索本地自建3万物质数据库，提供定性定量数据，可提供二级定性镜像评估图，交付：PDF格式报告，WIFF格式机器原始数据</span></p></td><td width="125" style="border-top: none; border-left: none; border-bottom: 1px solid rgb(244, 176, 131); border-right: 1px solid rgb(244, 176, 131); background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">20</span><span style=";font-family:'微软雅黑',sans-serif;color:black">个工作日</span></p></td></tr><tr><td width="103" style="border-right: 1px solid rgb(244, 176, 131); border-bottom: 1px solid rgb(244, 176, 131); border-left: 1px solid rgb(244, 176, 131); border-image: initial; border-top: none; padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">2</span></p></td><td width="255" style="border-top: none; border-left: none; border-bottom: 1px solid rgb(244, 176, 131); border-right: 1px solid rgb(244, 176, 131); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">靶向代谢组</span></p></td><td width="558" style="border-top: none; border-left: none; border-bottom: 1px solid rgb(244, 176, 131); border-right: 1px solid rgb(244, 176, 131); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">采用靶向代谢组方法进行检测并进行标准数据分析，包含能量代谢、氨基酸、胆汁酸、色氨酸（23种）、花生四烯酸、神经递质、黄酮类等，交付：PDF格式报告，WIFF格式机器原始数据</span></p></td><td width="125" style="border-top: none; border-left: none; border-bottom: 1px solid rgb(244, 176, 131); border-right: 1px solid rgb(244, 176, 131); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">20</span><span style=";font-family:'微软雅黑',sans-serif;color:black">个工作日</span></p></td></tr></tbody></table><p><br/></p></div> <div class="blank30"></div> <div class="blank30"></div> <!--自动调用自定义字段--> <div class="blank20"></div> <div 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class="plug-menu themeStyle" style="background-image:url('/template/D20170425/skin/images/wap/plug.png');border:0px;"> <li class="themeStyle out" style="background:"> <a href="tel:0592-5792117"><img src="/template/D20170425/skin/images/wap/plugmenu1.png"><label>电 话</label></a> </li> <li class="themeStyle out" style="background:"> <a href="sms:13599994885"><img src="/template/D20170425/skin/images/wap/plugmenu3.png"><label>短信</label></a> </li> <li class="themeStyle out" style="background:"> <a href="http://wpa.qq.com/msgrd?v=3&uin=525274299&site=qq&menu=yes" id="btn_fenxiang"><img src="/template/D20170425/skin/images/wap/plugmenu5.png"><label>QQ号码</label></a> </li> <li class="themeStyle out" style="background:"> <a href="/index.php?case=guestbook&act=index"><img src="/template/D20170425/skin/images/wap/plugmenu6.png"><label>留言</label></a> </li> </ul> </nav> </div> <div id="plug-wrap" style="display: none;" ></div> <script> $(function(){ $(".plug-menu").click(function(){ var li = $(this).parents('ul').find('li'); if(li.attr("class") == "themeStyle on"){ li.removeClass("themeStyle on"); li.addClass("themeStyle out"); }else{ li.removeClass("themeStyle out"); li.addClass("themeStyle on"); } }); }); </script> </div> <script type="text/javascript" src="/js/common.js"></script> <script> (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })(); </script> <!-- Bootstrap core JavaScript ================================================== --> <!-- Placed at the end of the document so the pages load faster --> <script src="/template/D20170425/skin/js/bootstrap.min.js"></script> <!-- Just to make our placeholder images work. 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