</noscript> <script> function stop(){ return false; } document.oncontextmenu=stop; </script> <script src="/template/D20170425/skin/js/jia.js"></script> <div class="hidden-xs"> <div class="logo-box"> <a href="/" ><img src="/upload/images/201705/14948387746153.png" alt="厦门生命互联科技有限公司官网（专注外泌体研究和应用）" /></a> </div> <div id="header"> <div class="menu-bg"></div> <div class="container"> <div class="top-fenxiang"> <span></span> <div class="fenxiang"> <a id="weixin" href="#"><img src="/template/D20170425/skin/images/base/ico3.png" width="33" height="27" alt="" /></a> <a href="#" onclick="jiathis_sendto('tsina');return false;"><img src="/template/D20170425/skin/images/base/ico4.png" width="33" height="27" alt="" /></a> <a href="http://www.genokon.com/#" onclick="jiathis_sendto('qzone');return false;"><img src="/template/D20170425/skin/images/base/ico5.png" width="33" height="27" alt="" /></a> </div> </div> <div class="sousuo10"> <form name='search' action="/index.php?case=archive&act=search" onsubmit="search_check();" method="post"> <div class="input-group"> <input type="text" name="keyword" class="form-control" placeholder="请输入查询信息！"> <span class="input-group-btn"> <input name='submit' type="submit" value="搜索" align="middle" class="btn btn-default" /> </span> </div><!-- /input-group --> </form> </div> <div class="menu"> <ul> <li class="one1"><a href="/">网站首页</a></li> <li class="one one2"> <a title="科研服务" href="list_112.htm" target="_blank">科研服务</a> <div class="menu2"> <ul> <li> <a title="分离鉴定" href="list_123.htm" target="_blank"> <div class="menu2-ico1"></div> <p>分离鉴定</p> </a> </li> <li> <a title="组学分析" href="list_124.htm" target="_blank"> <div class="menu2-ico2"></div> <p>组学分析</p> </a> </li> <li> <a title="功能验证" href="list_125.htm" target="_blank"> <div class="menu2-ico3"></div> <p>功能验证</p> </a> </li> </ul> </div></li> <li class="one one3"> <a title="技术开发" href="list_113.htm" target="_blank">技术开发</a> <div class="menu2"> <ul> <li> <a title="生产体系" href="list_129.htm" target="_blank"> <div class="menu2-ico1"></div> <p>生产体系</p> </a> </li> <li> <a title="评价模型" href="list_128.htm" target="_blank"> <div class="menu2-ico2"></div> <p>评价模型</p> </a> </li> <li> <a title="原料目录" href="list_130.htm" target="_blank"> <div class="menu2-ico3"></div> <p>原料目录</p> </a> </li> </ul> </div></li> <li class="one4"> <a title="项目管理" href="https://ss3616393.huoban.com/cs/8BT659K0" target="_blank">项目管理</a> <div class="menu2"> <ul> </ul> </div></li> <li class="one one5"> <a title="合作分享" href="list_115.htm" target="_blank">合作分享</a> <div class="menu2"> <ul> <li> <a title="科研申报" href="list_131.htm" target="_blank"> <div class="menu2-ico1"></div> <p>科研申报</p> </a> </li> <li> <a title="数据工具" href="list_132.htm" target="_blank"> <div class="menu2-ico2"></div> <p>数据工具</p> </a> </li> <li> <a title="技术分享" href="list_133.htm" target="_blank"> <div class="menu2-ico3"></div> <p>技术分享</p> </a> </li> </ul> </div></li> </ul> </div> </div> </div> <span id="aa" class="active"></span> <span id="bb"></span> <div id="leftnav"> <div class="leftnav-div"> <dl> <dt><a class="" href="/">网站首页</a></dt> </dl> <dl> <dt><a class=" on" target="">主营业务</a></dt> <dd> <div> <a title="科研服务" ">科研服务</a><br /> <span><a title="分离鉴定" href="list_123.htm">分离鉴定</a></span> <span><a title="组学分析" href="list_124.htm">组学分析</a></span> <span><a title="功能验证" href="list_125.htm">功能验证</a></span> </div> <div> <a title="技术开发" ">技术开发</a><br /> <span><a title="生产体系" href="list_129.htm">生产体系</a></span> <span><a title="评价模型" href="list_128.htm">评价模型</a></span> <span><a title="原料目录" href="list_130.htm">原料目录</a></span> </div> <div> <a title="合作分享" ">合作分享</a><br /> <span><a title="科研申报" href="list_131.htm">科研申报</a></span> <span><a title="数据工具" href="list_132.htm">数据工具</a></span> <span><a title="技术分享" href="list_133.htm">技术分享</a></span> </div> <div> <a title="项目管理" href="https://ss3616393.huoban.com/cs/8BT659K0"">项目管理</a><br /> </div> </dd> </dl> <dl> <dt><a class="" target="">关于我们</a></dt> <dd> <div> <a title="公司介绍" href="list_117.htm"">公司介绍</a><br /> </div> <div> <a title="公司环境" href="list_119.htm"">公司环境</a><br /> </div> <div> <a title="仪器平台" href="list_120.htm"">仪器平台</a><br /> </div> <div> <a title="科研环境" href="list_121.htm"">科研环境</a><br /> </div> <div> <a title="岗位招聘" href="list_122.htm"">岗位招聘</a><br /> </div> </dd> </dl> <dl> <dt><a class="" href="list_109.htm" target="">新闻动态</a></dt> </dl> <dl> <dt><a class="" href="/index.php?case=archive&act=list&catid=122" target="">招聘信息</a></dt> </dl> <dl> <dt><a class="" target="">更多</a></dt> <dd> <div> <a title="项目管理系统" href="list_136.htm"">项目管理系统</a><br /> </div> <div> <a title="新版项目系统" href="https://ss3616393.huoban.com/cs/8BT659K0"">新版项目系统</a><br /> </div> </dd> </dl> </div> </div> <script src="/template/D20170425/skin/js/index.js"></script> </div> <div class="hidden-sm hidden-md hidden-lg"> <!-- nav --> <nav class="navbar navbar-default navbar-fixed-top"> <div class="container-fluid"> <div class="navbar-header"> <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar" aria-expanded="false" aria-controls="navbar"> <span class="sr-only">厦门生命互联科技有限公司官网（专注外泌体研究和应用）</span> <span class="icon-bar"></span> <span class="icon-bar"></span> <span class="icon-bar"></span> </button> <!-- logo --> <a href="/" class="navbar-brand text-center"><img src="/upload/images/201705/14948387746153.png" class="img-responsive" alt="厦门生命互联科技有限公司官网（专注外泌体研究和应用）" /></a> <!-- logo end --> </div> <div id="navbar" class="navbar-collapse collapse"> <ul class="nav navbar-nav"> <li class="oen"><a href="/">网站首页</a></li> <li class="oen dropdown"> <a href="list_134.htm" data-toggle="dropdown" data-submenu="">主营业务<span class="caret"></span></a> <ul class="two dropdown-menu"> <li class="dropdown-submenu"> <a title="科研服务" href="list_112.htm">科研服务</a> <ul class="three dropdown-menu"> <li> <a title="分离鉴定" href="list_123.htm">分离鉴定</a> </li> <li> <a title="组学分析" href="list_124.htm">组学分析</a> </li> <li> <a title="功能验证" href="list_125.htm">功能验证</a> </li> </ul> </li> <li class="dropdown-submenu"> <a title="技术开发" href="list_113.htm">技术开发</a> <ul class="three dropdown-menu"> <li> <a title="生产体系" href="list_129.htm">生产体系</a> </li> <li> <a title="评价模型" href="list_128.htm">评价模型</a> </li> <li> <a title="原料目录" href="list_130.htm">原料目录</a> </li> </ul> </li> <li class="dropdown-submenu"> <a title="合作分享" href="list_115.htm">合作分享</a> <ul class="three dropdown-menu"> <li> <a title="科研申报" href="list_131.htm">科研申报</a> </li> <li> <a title="数据工具" href="list_132.htm">数据工具</a> </li> <li> <a title="技术分享" href="list_133.htm">技术分享</a> </li> </ul> </li> <li> <a title="项目管理" href="https://ss3616393.huoban.com/cs/8BT659K0">项目管理</a> </li> </ul> </li> <li class="oen dropdown"> <a href="list_108.htm" data-toggle="dropdown" data-submenu="">关于我们<span class="caret"></span></a> <ul class="two dropdown-menu"> <li> <a title="公司介绍" href="list_117.htm">公司介绍</a> </li> <li> <a title="公司环境" href="list_119.htm">公司环境</a> </li> <li> <a title="仪器平台" href="list_120.htm">仪器平台</a> </li> <li> <a title="科研环境" href="list_121.htm">科研环境</a> </li> <li> <a title="岗位招聘" href="list_122.htm">岗位招聘</a> </li> </ul> </li> <li class="oen"> <a href="list_109.htm">新闻动态</a> </li> <li class="oen"> <a href="/index.php?case=archive&act=list&catid=122">招聘信息</a> </li> <li class="oen dropdown"> <a href="list_135.htm" data-toggle="dropdown" data-submenu="">更多<span class="caret"></span></a> <ul class="two dropdown-menu"> <li> <a title="项目管理系统" href="list_136.htm">项目管理系统</a> </li> <li> <a title="新版项目系统" href="https://ss3616393.huoban.com/cs/8BT659K0">新版项目系统</a> </li> </ul> </li> </ul> </div> </div> </nav> </div> <div class="banner2"> <img src="/upload/images/201704/14934565309757.jpg" /> </div> <div class="title30"> <div class="container"> 质控分析<br /> <small>作者：<a>lifeint</a> 添加时间：2016-05-08 11:04:17 浏览：<script src="/index.php?case=archive&act=view_js&aid=225"></script></small> </div> </div> <hr /> <!-- 中部开始 --> <div class="container"> <!--用于打印和文字放大--> <div class="content_tools_box"> <p class="content_tools"> <a href="javascript:CallPrint('print');">打印本文</a> <a href="javascript:doZoom(14)">小</a> <a href="javascript:doZoom(18)">中</a> <a href="javascript:doZoom(20)">大</a> </p> <div class="clearfix"></div> <script src="/template/D20170425/skin/js/c_tool.js" type="text/javascript" ></script> </div> <!--/用于打印和文字放大--> <!-- 正文 --> <div class="lead content" id="print"> <div style="white-space: normal; list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: 19.2px; vertical-align: baseline;">服务项目</strong></span></div><p><br/></p><p style="white-space: normal; list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: 19.2px; vertical-align: baseline;"></strong></span></p><table><tbody><tr class="firstRow"><td width="75" style="border-top-width: 1px; border-top-color: rgb(237, 125, 49); border-bottom: 1px solid rgb(237, 125, 49); border-left-width: initial; border-left-color: initial; border-image: initial; border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务序号</span></strong></p></td><td width="255" style="border-top-width: 1px; border-top-color: rgb(237, 125, 49); border-left-width: initial; border-left-color: initial; border-bottom: 1px solid rgb(237, 125, 49); border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务内容</span></strong></p></td><td width="510" style="border-top-width: 1px; border-top-color: rgb(237, 125, 49); border-left-width: initial; border-left-color: initial; border-bottom: 1px solid rgb(237, 125, 49); border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务说明</span></strong></p></td><td width="142" style="border-top-width: 1px; border-top-color: rgb(237, 125, 49); border-left-width: initial; border-left-color: initial; border-bottom: 1px solid rgb(237, 125, 49); border-right: none; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">收费标准</span></strong></p></td><td width="142" style="border-width: 1px; border-color: rgb(237, 125, 49); border-right-style: solid; border-bottom-style: solid; border-image: initial; background: rgb(237, 125, 49); padding: 0px 7px;"><p style="text-align:center"><strong><span style=";font-family:'微软雅黑',sans-serif;color:black">服务周期</span></strong></p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">1</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">纯度检测（裂解法)</span></p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">检测triton-100裂解前后的外泌体颗粒浓度变化并分析外泌体的纯度值</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">500</span><span style="color:black">元/样</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">3</span><span style="color:black">个工作日</span></p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">2</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">纯度检测（染料法)</p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">采用膜染料（Evmembrane Green Stains）进行外泌体膜标记并分析外泌体的纯度值</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">500元/样</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">3个工作日</p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">3</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">电位检测（Zetaview）</span></p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">采用NTA（Zetaview）的方法检测外泌体的Zeta电位</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">300</span><span style="color:black">元/样</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">3</span><span style="color:black">个工作日</span></p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">4</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">渗透压检测（SMC 30D）</p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">采用渗透压摩尔浓度测定仪（SMC 30D）进行外泌体的渗透压检测</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">200元/样</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">3个工作日</p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">5</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">微生物检测（摇菌）</span></p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">采用摇菌24小时的方法检测外泌体是否存在染菌情况</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">200</span><span style="color:black">元/样</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">3</span><span style="color:black">个工作日</span></p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">6</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">支原体检测（qPCR）</p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">采用qPCR方法检测外泌体是否存在支原体感染</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">200元/样</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">3个工作日</p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">7</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">内毒素检测（鲎试剂）</span></p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">采用鲎试剂内毒素检测试剂盒检测外泌体的内毒素含量</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">200</span><span style="color:black">元/样</span></p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; background: rgb(251, 228, 213); padding: 0px 7px;"><p style="text-align:center"><span style="color:black">3</span><span style="color:black">个工作日</span></p></td></tr><tr><td width="75" style="border-width: 1px; border-right-style: solid; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-image: initial; padding: 0px 7px;"><p style="text-align:center"><span style=";font-family:'微软雅黑',sans-serif;color:black">8</span></p></td><td width="255" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">重金属检测（原子吸收）</p></td><td width="510" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">采用原子吸收方法检测外泌体是否含有铅镉铬汞砷5种元素</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">600元/样</p></td><td width="142" style="border-width: 1px; border-color: rgb(244, 176, 131); border-bottom-style: solid; border-right-style: solid; padding: 0px 7px;"><p style="text-align:center">5个工作日</p></td></tr></tbody></table><p style="white-space: normal; list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: 19.2px; vertical-align: baseline;"></strong></span><br/></p><p style="white-space: normal; list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: 19.2px; vertical-align: baseline;"><br/></strong></span></p><div style="white-space: normal; list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentcolor; font-size: 19.2px; vertical-align: baseline;">实验介绍</strong></span></div><p style="white-space: normal;"> <span style="font-family: 微软雅黑, "Microsoft YaHei";"> </span></p><p> 质量控制是外泌体研究中非常重要的一环，可以保证实验结果的准确性和可靠性。外泌体的纯度、微生物含量、内毒素含量都可能对下游进行组学、细胞、动物实验的数据产生影响，因此需要根据下游的具体用途来对外泌体进行相应的检测才能确保分离质量。</p><p style="text-align: center;"><br/></p><p style="white-space: normal;"><strong>1. 纯度检测</strong></p><p style="white-space: normal;"> 目前外泌体纯度的表征尚无统一的方法，调研文献发现方法主要有颗粒数/蛋白量、蛋白阴性指标检测、RNA片段分布分析、HPLC检测等方法来实现，经过实验测试数据发现，有两个方法在表征纯度方法数据的可信度较高：1.基于膜结构的生物特性，通过对比Triton X-100裂解外泌体颗粒前后的粒径数量变化，确定样本中含有脂膜结构的外泌体颗粒占比；2.通过膜染料标记方法，实现脂膜成分标记，通过纳米流式检测能够被染料标记发光的颗粒占比（该方法目前仅适用于动物细胞来源的外泌体）；</p><p style="text-align: center;"><img src="/upload/images/202304/16810379862581.jpg" title="16810379862581.jpg" alt="29.jpg"/></p><p style="white-space: normal;"><strong>2. 污染物分析</strong></p><p style="white-space: normal;"> 外泌体在制备过程中可能由于样本自身或者环境引入，导致可能存在细菌污染、支原体污染、内毒素含量偏高等问题，这些污染将导致下游实验存在数据偏差甚至是无法正常开展，因此对于外泌体的质量控制除了在制备环节尽可能保证无菌环境，还需要对制备好的外泌体进行相应的检测，符合要求后才能进行下游的检测或应用。</p><p style="white-space: normal; text-align: center;"><img src="/upload/images/202304/16810457273247.jpg" title="16810457273247.jpg" alt="30.jpg"/></p></div> <div class="blank30"></div> <div class="blank30"></div> <!--自动调用自定义字段--> <div class="blank20"></div> <div class="blank30"></div> <!-- 投票 --> <script src="/index.php?case=vote&act=view&aid=225"></script> <!-- 自定义表单开始 --> <!-- 自定义表单结束 --> <div class="blank60"></div> <!-- 上下页开始 --> <div id="page"> <strong>上一篇</strong>无 <div class="blank10"></div> <strong>下一篇</strong><a href="show_226.htm">基于CRISPR/CAS9系统的基因靶向技术</a> </div> <!-- 上下页结束 --> </div><!-- /container --> <!-- 相关文章开始 --> <!-- 相关文章结束 --> <div class="blank60"></div> <!-- 评论框开始 --> <link href="/template/D20170425/skin/css/comment.css" rel="stylesheet"> <div class="blank60"></div> <!-- 页面标题开始 --> <div class="title"> <h3><a>评论<small>COMMENT</small></a></h3> <p></p> <span>——</span> </div> <!-- 页面标题结束 --> <hr class="featurette-divider"> <div class="container"> <div class="blank30"></div> <form action="/index.php?case=comment&act=add" method="POST" name="comment_form" id="comment" class="wow fadeIn" data-wow-delay="0.6s"> <div class="comm"> <div class="blank10"></div> <input type="hidden" name="aid" value="225"/> 用户名 Name <div class="blank10"></div> <input type="text" name="username" class="comm_name" value="" /> <div class="blank10"></div> <div class="blank10"></div> 评论 Comment <div class="blank10"></div> <textarea name="content" id="textarea"></textarea> <div class="blank30"></div> <input name="submit" class="btn" value=" 提交 " type="submit" /> <div class="blank30"></div> <div class="comment_info"> <a rel="nofollow" href="/index.php?case=comment&act=list&aid=225">已有<span class="commentnumber">(0)</span>位网友发表评论 <strong>点击查看</strong></a> </div> </div><!-- /comm --> </form> <div class="blank60"></div> </div><!-- /container --> <!-- 评论框结束 --> <!-- 页底推荐图文产品开始 --> <hr class="featurette-divider"> <!-- 页面标题开始 --> <div class="title"> <h3 class="wow bounceInDown" data-wow-delay="0.5s"><a href="list_134.htm">主营业务<small></small></a></h3> <p class="wow bounceIn" data-wow-delay="0.5s"></p> <span>——</span> </div> <!-- 页面标题结束 --> <div class="container"> <div class="blank30"></div> <!-- 中部开始 --> <div class="row"> <div class="col-xs-12 col-sm-3 col-md-3 col-lg-3"> <p style="line-height:1.8;margin:0 0 20px;">微环DNA表达双靶向抗体的癌症免疫治疗技术成为继CAR-T技术(“嵌合抗原受体T细胞免疫疗法”)、TIL技术(“肿瘤渗润淋巴细胞技术)、免疫检查点抑制剂等技术之后又一项获得成功的新一代治疗技术。</p> <p><a title="" href="list_134.htm" class="content-bottom-list-more">Learn more</a></p> </div> <div class="col-xs-12 col-sm-9 col-md-9 col-lg-9"> <div class="row"> <div class="post-masonry col-xs-12 col-sm-6 col-md-4 col-lg-4"> <a title="植物外泌体测序" href="show_385.htm" class="img-auto"> <img class="img-responsive" alt="植物外泌体测序" src="/upload/images/202505/1747381045144.png" onerror='this.src="/images/nopic.gif"' /> <h4><font style="color:;">植物外泌体测序</font></h4> </a> </div> <div class="post-masonry col-xs-12 col-sm-6 col-md-4 col-lg-4"> <a title="植物外泌体" href="show_384.htm" class="img-auto"> <img class="img-responsive" alt="植物外泌体" src="/upload/images/202504/17443619689333.png" onerror='this.src="/images/nopic.gif"' /> <h4><font style="color:;">植物外泌体</font></h4> </a> </div> <div class="post-masonry col-xs-12 col-sm-6 col-md-4 col-lg-4"> <a title="基因组学" href="show_349.htm" class="img-auto"> <img class="img-responsive" alt="基因组学" src="/upload/images/202304/16807961175826.jpg" onerror='this.src="/images/nopic.gif"' /> <h4><font style="color:;">基因组学</font></h4> </a> </div> </div> </div> </div><!-- /row --> </div><!-- /container --> <div class="blank60"></div> <!-- 页底推荐图文产品结束 --> <div class="foot"> <div class="container"> <div class="foot-title"> <a href="list_110.htm" title="" target="_blank">合作单位</a> : </div> <div class="row"> <div class="foot1"> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位一" href="show_224.htm" target="_blank"><img alt="合作单位一" src="/upload/images/201711/1510227719947.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位一" target="_blank"><img alt="合作单位一" src="/upload/images/201711/1510227719947.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位二" href="show_223.htm" target="_blank"><img alt="合作单位二" src="/upload/images/201712/15124351904626.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位二" target="_blank"><img alt="合作单位二" src="/upload/images/201712/15124351904626.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-xs-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位三" href="show_222.htm" target="_blank"><img alt="合作单位三" src="/upload/images/201712/15124352179401.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位三" target="_blank"><img alt="合作单位三" src="/upload/images/201712/15124352179401.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-sm-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位四" href="show_221.htm" target="_blank"><img alt="合作单位四" src="/upload/images/201712/15124352349918.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位四" target="_blank"><img alt="合作单位四" src="/upload/images/201712/15124352349918.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-xs-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位五" href="show_220.htm" target="_blank"><img alt="合作单位五" src="/upload/images/201712/15124352556122.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位五" target="_blank"><img alt="合作单位五" src="/upload/images/201712/15124352556122.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-md-block visible-md-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位六" href="show_219.htm" target="_blank"><img alt="合作单位六" src="/upload/images/201712/15124352725994.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位六" target="_blank"><img alt="合作单位六" src="/upload/images/201712/15124352725994.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-xs-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位七" href="show_218.htm" target="_blank"><img alt="合作单位七" src="/upload/images/201711/15102278126595.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位七" target="_blank"><img alt="合作单位七" src="/upload/images/201711/15102278126595.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位八" href="show_217.htm" target="_blank"><img alt="合作单位八" src="/upload/images/201711/15102278285752.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位八" target="_blank"><img alt="合作单位八" src="/upload/images/201711/15102278285752.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-xs-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位九" href="show_216.htm" target="_blank"><img alt="合作单位九" src="/upload/images/201711/15102278440633.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位九" target="_blank"><img alt="合作单位九" src="/upload/images/201711/15102278440633.png" onerror='this.src="/images/nopic.gif"' /></a> </div> <div class="clearfix visible-sm-block"></div> <div class="col-sm-4 col-md-4 col-xs-6 foot1-item"> <!-- <a title="合作单位十" href="show_215.htm" target="_blank"><img alt="合作单位十" src="/upload/images/201711/15102278572726.png" onerror='this.src="/images/nopic.gif"' /></a>--> <a title="合作单位十" target="_blank"><img alt="合作单位十" src="/upload/images/201711/15102278572726.png" onerror='this.src="/images/nopic.gif"' /></a> </div> </div> </div> <div class="row"> <div class="foot2"> <div class="col-lg-5 col-md-4 col-sm-4"> <div class="foot-title"> 联系我们: </div> <ul> <li class="foot-ico1">厦门市海沧区翁角西路生物医药产业园B2号楼1001-1002室</li> <li class="foot-ico2">0592-5792117 微信18965129960 </li> <li class="foot-ico3">info@lifeint.cn </li> </ul> </div> <div class="col-lg-5 col-md-5 col-sm-5"> <div class="foot-map"> <script type="text/javascript" src="http://api.map.baidu.com/api?key=&v=1.1&services=true"></script> <div style="width:100%;height:100%;max-width:100%;margin:0 auto;" id="dituContent"></div> <script type="text/javascript"> //创建和初始化地图函数： function initMap(){ createMap();//创建地图 setMapEvent();//设置地图事件 addMapControl();//向地图添加控件 addMarker();//向地图中添加marker } //创建地图函数： function createMap(){ var map = new BMap.Map("dituContent");//在百度地图容器中创建一个地图 var point = new BMap.Point(117.962931,24.530067);//定义一个中心点坐标 map.centerAndZoom(point,12);//设定地图的中心点和坐标并将地图显示在地图容器中 window.map = map;//将map变量存储在全局 } //地图事件设置函数： function setMapEvent(){ map.enableDragging();//启用地图拖拽事件，默认启用(可不写) map.enableScrollWheelZoom();//启用地图滚轮放大缩小 map.enableDoubleClickZoom();//启用鼠标双击放大，默认启用(可不写) map.enableKeyboard();//启用键盘上下左右键移动地图 } //地图控件添加函数： function addMapControl(){ //向地图中添加缩放控件 var ctrl_nav = new BMap.NavigationControl({anchor:BMAP_ANCHOR_TOP_RIGHT,type:BMAP_NAVIGATION_CONTROL_SMALL}); map.addControl(ctrl_nav); } //标注点数组 var markerArr = [{title:"厦门生命互联科技有限公司",content:"厦门海沧生物医药产业园",point:"116.47033|39.919009",isOpen:0,icon:{w:21,h:21,l:0,t:0,x:6,lb:5}} ]; //创建marker function addMarker(){ for(var i=0;i<markerArr.length;i++){ var json = markerArr[i]; var p0 = json.point.split("|")[0]; var p1 = json.point.split("|")[1]; var point = new BMap.Point(p0,p1); var iconImg = createIcon(json.icon); var marker = new BMap.Marker(point,{icon:iconImg}); var iw = createInfoWindow(i); var label = new BMap.Label(json.title,{"offset":new BMap.Size(json.icon.lb-json.icon.x+10,-20)}); marker.setLabel(label); map.addOverlay(marker); map.openInfoWindow(iw, map.getCenter()); label.setStyle({ borderColor:"#808080", color:"#333", cursor:"pointer" }); (function(){ var index = i; var _iw = createInfoWindow(i); var _marker = marker; _marker.addEventListener("click",function(){ this.openInfoWindow(_iw); }); _iw.addEventListener("open",function(){ _marker.getLabel().hide(); }) _iw.addEventListener("close",function(){ _marker.getLabel().show(); }) label.addEventListener("click",function(){ _marker.openInfoWindow(_iw); }) if(!!json.isOpen){ label.hide(); _marker.openInfoWindow(_iw); } })() } } //创建InfoWindow function createInfoWindow(i){ var json = markerArr[i]; var iw = new BMap.InfoWindow("<b class='iw_poi_title' title='" + json.title + "'>" + json.title + "</b><div class='iw_poi_content'>"+json.content+"</div>"); return iw; } //创建一个Icon function createIcon(json){ var icon = new BMap.Icon("/images/map/us_mk_icon.png", new BMap.Size(json.w,json.h),{imageOffset: new BMap.Size(-json.l,-json.t),infoWindowOffset:new BMap.Size(json.lb+5,1),offset:new BMap.Size(json.x,json.h)}) return icon; } initMap();//创建和初始化地图 </script> </div> </div> <div class="col-lg-2 col-md-3 col-sm-3"> <div class="foot-weixin"> <img src="/upload/images/201706/14963135840929.jpg" width="157" height="158" alt="官方微信号" /> <p>官方微信号</p> </div> </div> </div> </div> </div> </div> <div class="copyright"> <div class="container"> Copyright ©<a href="http://www.lifeint.cn" target="_blank">厦门生命互联科技有限公司官网（专注外泌体研究和应用）</a> <a href="https://beian.miit.gov.cn/" target="_blank">闽ICP备15016135号-1</a> </div> </div> <div id="outer-w"> <div id="outer" > <div id="middle"> <div id="inner" class="inner"> <div class="inner1"> <div class="weixin-title">分享到微信朋友圈</div> <div class="weixin-pic"> <img src="/upload/images/201706/14963135840929.jpg" width="200" height="200" alt="" /> </div> <div class="weixin-title">打开微信，点击底部的“发现”，使用 “扫一扫” 即可将网页分享到我的朋友圈。 如何使用？</div> <div id="close"></div> </div> </div> </div> </div> <div id="bg-black"></div> </div> <div class="leftnav-bg"></div> <div class="leftnav-bg2"></div> <script src="/template/D20170425/skin/js/jQuery.autoIMG.js"></script> <script type="text/javascript"> <!-- jQuery(function ($) { $('.lead p').autoIMG(); }); //--> </script> <!-- search --> <div class="container-fluid"> <div class="modal fade bs-example-modal-lg-search" tabindex="-1" role="dialog" aria-labelledby="myLargeModalLabel"> <div class="modal-dialog modal-lg"> <div class="modal-content"> <div class="row"> <form name='search' action="/index.php?case=archive&act=search" onsubmit="search_check();" method="post"> <div class="col-lg-1"></div> <div class="col-lg-10"> <div class="input-group"> <input type="text" name="keyword" class="form-control" placeholder="请输入查询信息！"> <span class="input-group-btn"> <button class="btn btn-default" name='submit' type="submit">Go!</button> </span> </div> </div> <div class="col-lg-1"></div> </form> </div> </div> </div> </div> <!-- search end --> <div class="servers"> <!--[if (gte IE 7)|!(IE)]><!--> <!-- 在线客服 --> <![endif]--> <!-- 短信 --> </div> <div class="servers-wap"> <style type="text/css"> #plug-wrap { position: fixed; top: 0; left: 0; width: 100%; height: 100%; background: rgba(0, 0, 0, 0); z-index:800; } .top_bar { position:fixed; bottom:0; right:0px; z-index:900; -webkit-tap-highlight-color: rgba(0, 0, 0, 0); font-family: Helvetica, Tahoma, Arial, Microsoft YaHei, sans-serif; } .plug-menu { -webkit-appearance:button; display:inline-block; width:36px; height:36px; border-radius:36px; position: absolute; bottom:17px; right: 17px; z-index:999; box-shadow: 0 0 0 4px #FFFFFF, 0 2px 5px 4px rgba(0, 0, 0, 0.25); -webkit-transition: -webkit-transform 200ms; -webkit-transform:rotate(1deg); color:#fff; background-image:url('/template/D20170425/skin/images/wao/plug.png'); background-repeat: no-repeat; -webkit-background-size: 80% auto; background-size: 80% auto; background-position: center center; } .plug-menu:before { font-size:20px; margin:9px 0 0 9px; } .plug-menu:checked { -webkit-transform:rotate(135deg); } .top_menu>li { width: 32px; height:32px; border-radius:32px; box-shadow: 0 0 0 3px #FFFFFF, 0 2px 5px 3px rgba(0, 0, 0, 0.25); position:absolute; bottom:0; right:0; margin-bottom: 20px; margin-right:20px; z-index:900; -webkit-transition: -webkit-transform 200ms; } .top_menu>li a { color:#fff; font-size:20px; display: block; height: 100%; line-height: 33px; text-align: center; } .top_menu>li>a label{ display:none; } .top_menu>li a img { display: block; width: 22px; height: 22px; text-indent: -999px; position: absolute; top: 50%; left: 50%; margin-top: -11px; margin-left: -11px; } .top_menu>li.on:nth-of-type(1) { -webkit-transform: translate(-0, -100px) rotate(720deg); } .top_menu>li.on:nth-of-type(2) { -webkit-transform: translate(-47px, -81px) rotate(720deg); } .top_menu>li.on:nth-of-type(3) { -webkit-transform: translate(-81px, -45px) rotate(720deg); } .top_menu>li.on:nth-of-type(4) { -webkit-transform: translate(-100px, 0) rotate(720deg); } #sharemcover { position: fixed; top: 0; left: 0; width: 100%; height: 100%; background: rgba(0, 0, 0, 0.7); display: none; z-index: 20000; } #sharemcover img { position: fixed; right: 18px; top: 5px; width: 260px; height: 180px; z-index: 20001; border:0; } </style> <div class="top_bar" style="-webkit-transform:translate3d(0,0,0)"> <nav> <ul id="top_menu" class="top_menu"> <input type="checkbox" id="plug-btn" class="plug-menu themeStyle" style="background-image:url('/template/D20170425/skin/images/wap/plug.png');border:0px;"> <li class="themeStyle out" style="background:"> <a href="tel:0592-5792117 微信18965129960"><img src="/template/D20170425/skin/images/wap/plugmenu1.png"><label>电 话</label></a> </li> <li class="themeStyle out" style="background:"> <a href="sms:13599994885"><img src="/template/D20170425/skin/images/wap/plugmenu3.png"><label>短信</label></a> </li> <li class="themeStyle out" style="background:"> <a href="http://wpa.qq.com/msgrd?v=3&uin=525274299&site=qq&menu=yes" id="btn_fenxiang"><img src="/template/D20170425/skin/images/wap/plugmenu5.png"><label>QQ号码</label></a> </li> <li class="themeStyle out" style="background:"> <a href="/index.php?case=guestbook&act=index"><img src="/template/D20170425/skin/images/wap/plugmenu6.png"><label>留言</label></a> </li> </ul> </nav> </div> <div id="plug-wrap" style="display: none;" ></div> <script> $(function(){ $(".plug-menu").click(function(){ var li = $(this).parents('ul').find('li'); if(li.attr("class") == "themeStyle on"){ li.removeClass("themeStyle on"); li.addClass("themeStyle out"); }else{ li.removeClass("themeStyle out"); li.addClass("themeStyle on"); } }); }); </script> </div> <script type="text/javascript" src="/js/common.js"></script> <script> (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })(); </script> <!-- Bootstrap core JavaScript ================================================== --> <!-- Placed at the end of the document so the pages load faster --> <script src="/template/D20170425/skin/js/bootstrap.min.js"></script> <!-- Just to make our placeholder images work. Don't actually copy the next line! --> <script src="/template/D20170425/skin/js/holder.min.js"></script> <!-- IE10 viewport hack for Surface/desktop Windows 8 bug --> <script src="/template/D20170425/skin/js/ie10-viewport-bug-workaround.js"></script> <script src="/template/D20170425/skin/js/bootstrap-submenu.js"></script> <script src="/template/D20170425/skin/js/docs.js"></script> <!--[if lt IE 9]><!--> <script src="/template/D20170425/skin/js/ie/html5shiv.min.js"></script> <script src="/template/D20170425/skin/js/ie/respond.min.js"></script> <![endif]--> <link rel="stylesheet" href="/template/D20170425/skin/css/animate.min.css" /> <script src="/template/D20170425/skin/js/wow.min.js"></script> <script> if (!(/msie [6|7|8|9]/i.test(navigator.userAgent))){ new WOW().init(); }; </script> </body> </html>