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class="container"> 超速离心<br /> <small>作者：<a></a> 添加时间：2022-11-23 14:38:33 浏览：<script src="/index.php?case=archive&act=view_js&aid=322"></script></small> </div> </div> <hr /> <!-- 中部开始 --> <div class="container"> <!--用于打印和文字放大--> <div class="content_tools_box"> <p class="content_tools"> <a href="javascript:CallPrint('print');">打印本文</a> <a href="javascript:doZoom(14)">小</a> <a href="javascript:doZoom(18)">中</a> <a href="javascript:doZoom(20)">大</a> </p> <div class="clearfix"></div> <script src="/template/D20170425/skin/js/c_tool.js" type="text/javascript" ></script> </div> <!--/用于打印和文字放大--> <!-- 正文 --> <div class="lead content" id="print"> <div style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; white-space: normal; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; color: rgb(0, 0, 0); font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; font-size: 19.2px; vertical-align: baseline;">1.实验介绍</strong></span></div><p style="white-space: normal;"><span style="color: rgb(0, 0, 0);"> </span></p><div style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; white-space: normal; background-color: rgb(255, 255, 255);"><p><span style="color: rgb(0, 0, 0); font-family: 微软雅黑, "Microsoft YaHei";"> </span>外泌体（exosomes）是一种能被机体内大多数细胞分泌的直径大约为30-150nm的具有脂质双层膜的微小囊泡。它广泛存在并分布于各种体液中，携带和传递重要的信号分子，形成了一种全新的细胞间信息传递系统，影响细胞的生理状态并与多种疾病的发生与进程密切相关。2013年，发现细胞囊泡转运机制的科学家们，荣获了当年的诺贝尔生理学或医学奖。作为人体内的一类重要囊泡，外泌体自2013年起逐步成为科研热点。<br/> 超速离心分离提纯外泌体并由电镜、粒径、蛋白做表征检测的方法是目前主流期刊文章最推崇的手段，国际细胞外囊泡研究协会（ISEV）也发布了囊泡研究所需的最少实验要求：<br/>1.蛋白鉴定：Western Blot或流式细胞术<br/>2.粒径分析：动态光散射(DLS)或纳米颗粒跟踪分析(NTA)<br/>3.形态表征：透射电子显微镜（TEM）或原子力显微镜（AFM）<span style="color: rgb(0, 0, 0); font-family: 微软雅黑, "Microsoft YaHei";"></span></p><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; color: rgb(0, 0, 0); font-size: medium; vertical-align: baseline;"></span></div><p style="white-space: normal;"><span style="color: rgb(0, 0, 0);"> </span></p><p style="white-space: normal;"><span style="color: rgb(0, 0, 0);"> </span></p><div style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; white-space: normal; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; color: rgb(0, 0, 0); font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; font-size: 19.2px; vertical-align: baseline;">2.实验流程</strong></span></div><p style="white-space: normal;"><span style="color: rgb(0, 0, 0);"> </span></p><p style="text-align: center;"><img width="1190" height="614" style="width: 682px; height: 378px;" alt="image.png" src="/upload/images/201905/15577993943052.png"/></p><p style="white-space: normal;"><span style="color: rgb(0, 0, 0);"> </span></p><p style="white-space: normal;"><br/></p><div style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; line-height: 28.8px; font-family: 微软雅黑; vertical-align: baseline; white-space: normal; background-color: rgb(255, 255, 255);"><span style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; color: rgb(0, 0, 0); font-size: larger; vertical-align: baseline;"><strong style="list-style: none; margin: 0px; padding: 0px; outline: 0px; border: 0px currentColor; border-image: none; font-size: 19.2px; vertical-align: baseline;">3.实验结果</strong></span></div><p style="white-space: normal;"><span style="color: rgb(0, 0, 0);"> </span></p><p style="text-align: center;"><img width="1305" height="534" style="width: 666px; height: 277px;" alt="image.png" src="/upload/images/201905/15577994226141.png"/></p></div> <div class="blank30"></div> <div class="blank30"></div> <!--自动调用自定义字段--> <div class="blank20"></div> <div class="blank30"></div> <!-- 投票 --> <script src="/index.php?case=vote&act=view&aid=322"></script> <!-- 自定义表单开始 --> <!-- 自定义表单结束 --> <div class="blank60"></div> <!-- 上下页开始 --> <div id="page"> <strong>上一篇</strong><a href="show_291.htm">无菌质控</a> <div class="blank10"></div> <strong>下一篇</strong>无 </div> <!-- 上下页结束 --> </div><!-- /container --> <!-- 相关文章开始 --> <div class="blank60"></div> <!-- 页面标题开始 --> <div class="title"> <h3><a><small>相关内容</small></a></h3> <p></p> <span>——</span> </div> <!-- 页面标题结束 --> <hr class="featurette-divider"> <div class="container list-container"> <!-- 内容列表开始 --> <div class="news-list list-border list-border-w"> <div class="list-date"> <span>23</span> <p>2022-11</p> </div> <h4><a title="超速离心" href="show_322.htm" target="_blank">超速离心</a></h4> <p>外泌体（exosomes）是一种能被机体内大多数细胞分泌的直径大约为30-150nm的具有脂质双层膜的微小囊泡。它广泛存在并分布于各种体液中，携带和传递重要的信号分子，形成了一种全新的细胞间信息传递系统，影响细胞的生理状态并与多种疾病的发生与进程密切相关。2013年，发现细胞囊泡… <a href="show_322.htm" target="_blank">[了解更多]</a></p> </div> <!-- 内容列表结束 --> </div> <!-- 相关文章结束 --> <div class="blank60"></div> <!-- 评论框开始 --> <link href="/template/D20170425/skin/css/comment.css" rel="stylesheet"> <div class="blank60"></div> <!-- 页面标题开始 --> <div class="title"> <h3><a>评论<small>COMMENT</small></a></h3> <p></p> <span>——</span> </div> <!-- 页面标题结束 --> <hr class="featurette-divider"> <div class="container"> <div class="blank30"></div> <form action="/index.php?case=comment&act=add" method="POST" name="comment_form" id="comment" class="wow fadeIn" data-wow-delay="0.6s"> 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